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Tail Length Tail AreaThe length of the comet tail is computed as the The total area of the comet tail is the number ofcomet head diameter subtracted from the overall pixels in the comet tail. All data collected from this and subsequent images will be appended to the same data file. A graphicrepresentation is superimposed on the digital image. Repeat steps 2 and 3 to load another bitmap image into the software. CometScore requires a Windows Bitmap image where all comets are oriented as head on the leftand tail on the right. Use the cutoff bar to eliminate the background from the field of view.

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Comet Length Comet HeightThe overall length of a comet is the number of The overall height of a comet is the number ofpixels in the horizontal direction of the comet.

Click the left mouse button over the field of view and drag a rectangle around a single comet. A graphicrepresentation is superimposed on the digital image.

A graphic representation is superimposed onthe digital image. Total Tail Intensity Mean Tail IntensityThe total comet tail intensity is the sum of all pixel The mean autofomet tail intensity is the average of allintensity values in comet tail.

Auticomet graphic representation issuperimposed on the digital image. Comet AreaThe total area of a comet is the number of pixelsin the comet. Create your own flipbook.

For a description of each equation used in the CometScore software, see Appendix: Mean Comet Intensity The mean comet intensity is the average of all pixel intensity values in the comet. EquationsScoring equations for the CometScore software are defined below.

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Algorithms are based on theseequations. This will isolate the comets from background noise during analysis and produce more reliable metrics. Repeat steps 4 and 5 until every isolated comet is scored in the field of view. CometScore requires a Windows Bitmap image where all comets are oriented as head on the leftand tail on the right. Head Diameter Head AreaThe diameter of the autoclmet is the number of pixels The total area of the comet head is the number ofin horizontal direction of the comet head.

Comet Intensity The total comet intensity is the sum of all pixel intensity values in the comet. Read the Text Version. You can publish your book online for free in a few minutes! At this point, the program will prompt you for autoxomet output filename.

CometScore Tutorial - Automatic Comet Assay

Use the cutoff bar to eliminate the background from the field of view. Click File - Open to load a bitmap into the software. Make sure that the rectangle does not overlap other comets in the field.

When all data has been collected, the file may be opened in any text viewer. To begin, double-click on the CometScore.

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All data collected from this and subsequent images will be appended to the same data file. Move the mouse over the head of the currently selected comet and left click on the center of the head.

To begin a new data file, you must close and reopen CometScore. Head Intensity IntegralThe comet head intensity integral is the vertical summationof all pixels intensities in the comet head, calculated bymirroring the comet intensity integral about a designatedcenter point. This occurs only one time during the program's lifecycle. Tail Length Tail AreaThe length of the comet tail is computed as the The total area of the comet tail is the number ofcomet head diameter subtracted from the overall pixels in the comet tail.

The data is tab- delimited and is more easily viewed in a third party spreadsheet program, such as Microsoft Excel.

Repeat steps 2 and 3 to load another bitmap image into the software. This tutorial will demonstrate how to acquire comet metrics from image data with CometScore.

Total Head Intensity Mean Head IntensityThe total comet head intensity is the sum of all The mean comet head intensity is the average ofpixel intensity values in comet head.